Developing liveShigellavaccines using λ Red recombineering
نویسندگان
چکیده
منابع مشابه
Recombineering
Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phage-derived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first developed in E. coli but has since been adapted for use in other bacteria. It is now widely used in a number of different systems for a variety of ...
متن کاملBacteriophage recombineering in the lytic state using the lambda red recombinases
Bacteriophages, the historic model organisms facilitating the initiation of molecular biology, are still important candidates of numerous useful or promising biotechnological applications. Development of generally applicable, simple and rapid techniques for their genetic engineering is therefore a validated goal. In this article, we report the use of bacteriophage recombineering with electropor...
متن کاملManipulating the Mouse Genome Using Recombineering
Animal models are routinely used to understand the etiology of human genetic disorders, as well as to develop preclinical models to test the efficacy of novel therapies. Although there are speciesspecific variations based on gene function studies, the mouse is the most reliable and commonly used model system, mainly because of its genetic and physiological similarity to humans. In addition, adv...
متن کاملBacterial Artificial Chromosome Mutagenesis Using Recombineering
Gene expression from bacterial artificial chromosome (BAC) clones has been demonstrated to facilitate physiologically relevant levels compared to viral and nonviral cDNA vectors. BACs are large enough to transfer intact genes in their native chromosomal setting together with flanking regulatory elements to provide all the signals for correct spatiotemporal gene expression. Until recently, the u...
متن کاملSite-Directed Mutagenesis Using Oligonucleotide-Based Recombineering
Methods enabling mutational analysis of distinct chromosomal locations, like site-directed mutagenesis, insertion of foreign sequences or in-frame deletions, have become of fast growing interest since complete bacterial genome sequences became available. Various approaches have been described to modify any nucleotide(s) in almost any manner. Some genetic engineering technologies do not rely on ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Immunology & Medical Microbiology
سال: 2006
ISSN: 0928-8244,1574-695X
DOI: 10.1111/j.1574-695x.2006.00118.x